Guaraná a Caffeine-Rich Food Increases Oxaliplatin Sensitivity of Colorectal HT-29 Cells by Apoptosis Pathway Modulation: We investigated the in vitro effects of guaraná and its main metabolites (caffeine, theobromine and catechin) on cytotoxicity and cell proliferation on colorectal cancer (CRC) line HT-29 cells and on oxaliplatin sensitivity. The cells were exposed to different concentrations of guaraná extract with and without oxaliplatin. The concentrations of bioactive molecules were also estimated considering their potential proportion on guaraná hydro-alcoholic extract. Apoptosis effect was analyzed by annexin V quantification using flow cytometry, while apoptosis pathway gene modulation (p53, Bax/Bcl-2 genes ratio, caspases 8 and 3) was determined by qRT-PCR analysis. Cells exposed to guaraná at a concentration of 100 μg/mL presented a similar cytotoxic effect as HT-29 cells treated with oxaliplatin and did not affect the sensitivity of the drug. Guaraná presented cell anti-proliferative effect and increased anti-proliferative oxaliplatin sensitivity at all concentrations tested here. Guaraná was able to induce apoptosis and up-regulate the p53 and Bax/Bcl-2 genes.
http://ift.tt/2dhPNj3
Ιατρική : Τα αισθητικά συστήματα της όρασης,ακοής,αφής,γεύσης και όσφρησης.
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
Δημοφιλείς αναρτήσεις
-
Publication date: Available online 8 April 2017 Source: European Journal of Vascular and Endovascular Surgery Author(s): M. Venermo, K. Ma...
-
http://ift.tt/2pB94zK http://ift.tt/2pQ5J0M
-
AbstractBackground Early experience with transcatheter aortic valve replacement (TAVR) within failed bioprosthetic surgical aortic valves h...
-
Related Articles Views of Japanese medical students on the work-life balance of female physicians. Int J Med Educ. 2017 May 11;8:165-...
-
Soft tissue reactions to paraffin include inflammation, fibrosis, disfigurement, and granulomatous inflammation with foreign body giant cell...
-
Abstract Parkinson's disease (PD) is a progressive neurodegenerative disorder that is diagnosed largely on clinical grounds due to cha...
-
Abstract Background Previous studies have revealed the importance of microRNAs' (miRNAs) function as biomarkers in diagnosing human bl...
-
Publication date: July 2016 Source: American Journal of Orthodontics and Dentofacial Orthopedics, Volume 150, Issue 1 Author(s): Gökhan Se...
-
Typically, the subjective pleasantness of an odor decreases after it has been repeatedly smelled. This study investigates how the pleasantne...
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου