Simultaneous Separation and Determination of 15 Organic UV Filters in Sunscreen Cosmetics by HPLC-ESI-MS/MS

Abstract

Objective

A comprehensive methodology for the simultaneous determination of 15 multiclass organic UV filters in sunscreen cosmetics was developed using high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS).

Methods

Sunscreen cosmetics of various matrices, such as toning lotion, emulsion, cream, and lipstick, were analyzed. Ultrasound-assisted extraction (UAE) was utilized as the extraction technique for sample preparation. The 15 UV filters were chromatographically separated by two groups of mobile phase system on an XBridge C₁₈ analytical column (150 mm × 2.1 mm I.D., 3.5 μm particle size) and quantified using HPLC-ESI-MS/MS. The quantitation was performed using the external calibration method.

Results

The established method was validated in terms of linearity, sensitivity, specificity, accuracy, stability, intra- and inter-day precisions, recovery, and matrix effect. The method was also applied for the determination of UV filters in commercial sunscreen cosmetics.

Conclusion

The experimental results demonstrated that the developed method was accurate, rapid, sensitive, and can be used for the analytical control of sunscreen cosmetics.

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