TGF-β1 promotes human gastric carcinoma SGC7901 cells invasion by inducing autophagy.

TGF-β1 promotes human gastric carcinoma SGC7901 cells invasion by inducing autophagy.

Eur Rev Med Pharmacol Sci. 2017 Mar;21(5):1013-1019

Authors: Shen J, Zhao DS, Li MZ

Abstract
OBJECTIVE: To investigate the role of TGF-b1 in autophagy and invasion ability in human gastric carcinoma cell line SGC7901.
MATERIALS AND METHODS: Cultured SGC7901 cells were treated with different concentrations of TGF-b1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-b1 on invasion ability of SGC7901 cells was detected with transwell method.
RESULTS: The results demonstrated that TGF-b1 was able to induced autophagy of SGC7901 cells in a dose-dependent manner. Autophagy inhibitor 3-MA could inhibit TGF-b1 upregulated autophagy. Furthermore, TGF-b1 significantly enhances the invasion ability of SGC7901 cells. However, autophagy inhibitor 3-MA could effectively reverse this process.
CONCLUSIONS: TGF-b1 enhances SGC7901 cells migration by inducing autophagy.

PMID: 28338195 [PubMed - in process]

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