Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by LPS

Abstract

Aim

To investigate the role of LPS in the odontoclast differentiation of MDPC-23 cells. It was hypothesized that MDPC 23 odontoblast-like cells may function as odontoclasts under the influence of LPS.

Methodology

MDPC-23 cells were cultured in the presence of 0.1 or 1 μg/mL LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast-like function of the induced cells. In additional, the related protein expression was confirmed by western blotting and ELISA. An unpaired Student's t-test and one-way ANOVA were used in statistical analysis.

Results

TRAP-positive cells, which are multinucleated, on the dentine slice were significantly increased in 1 μg/mL LPS-induced cells (p < 0.05). Osteoclast-specific proteins such as TRAP cathepsin K and Rac1 were up-regulated in the 1 μg/mL LPS-treated cells (p < 0.05), while the expression of marker proteins of the RANKL-RANK signaling pathway (RANKL, RANK and TRAF6) in the induced cells was not significantly changed (p > 0.05). ROS production was observed in the 1 μg/mL LPS treatment group (p < 0.05), but no significant differences were observed in the level of RANKL in the cell supernatant between the LPS-treated group and the control group (p > 0.05).

Conclusions

1 μg/mL LPS might induce odontoblast-like MDPC-23 cells to generate odontoclast-like cells or to function as odontoclasts. The data might provide a new explanation for the precursors of odontoclasts and root resorption.

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